Real-time imaging of lung slices

Septation, cell clustering and epithelial cell migration of epithelial cells to existing airspaces in P3 PCLS. Epithelial cell dynamics in P3 PCLS. EpCAM-FITC (green) and SiR-DNA (magenta) labelled P3 PCLS imaged for 12 hours 45 minutes at 15 minute intervals. Red arrows indicate migrating septa, as one existing airway subdivides into two (a1-a2 and a3-a4). Blue circles indicate areas where cell clustering can be seen. Migrating epithelial cells intercalate with existing alveolar wall epithelial cells (yellow arrows) around two airspace, a5 and a6.
Epithelial cells integrate into an existing airspace EpCAM-FITC (red) and SiR-DNA (cyan) labelled P3 PCLS imaged for 14 hours 15 minutes at 15 minute intervals. Epithelial cells 1,2 and 3 (green arrows) migrate towards an existing alveolar airspace (white circle).
Visualisation of the epithelium and capillary network in P3 PCLS. EpCAM-FITC (green) and PECAM-Alexa 647 (red) labelled P3 PCLS imaged for 12 hours 30 minutes at 15 minute intervals. Both EpCAM positive epithelial and PECAM positive endothelial cells can be seen in an extending septum during septation.
Control and blebbistatin treated P3 PCLS EpCAM-FITC (green) and SiR-DNA (magenta) labelled P3 PCLS treated with DMSO control media (A), imaged for 14 hours at 15 min intervals or 50µM blebbistatin containing media (B), imaged for 14 hours 15 minute intervals. a= airspaces.

Liverpool Research Conference

On 29th February Charlotte attended the 5th Annual National Liverpool Research Conference. This student led initiative is a one day meeting specifically aimed to encourage medical students to get involved in research. The day began with several talks by senior clinical and basic research scientists on a variety of topics such as ‘engineering an artificial womb’ and Charlotte’s talk ‘towards lung regeneration:tools and mechanisms’. There was also a poster and oral presentation competition, which showcased the many different projects that students have been working on. Participants were also able to attend workshops to learn more about routes into clinical academic research. A huge thanks to all the organisers and attendees for a thoroughly enjoyable day.

Meeting was held at the Liverpool Medical Institute opposite the catholic cathedral (pictured).

New Book on stem-cell therapies

For a really usefull up to date look at stem-cell based therapies, take a look at this book which has just been published. The chapter on ‘stem cell delivery systems and devices’ was co-authored by Sally Kim, a current ERS/EMBO fellow in the lab.

Mechanical Forces in Development

EMBO-EMBL Symposia Logo

Sek Shir recently presented her interesting new data at a poster entitled “Disruption of The Planar Cell Polarity (PCP) Component Vangl2 Alters Cell Mechanics in Loop-tail Mice” at the EMBO/EMBL symposium in Heidelberg, Germany between 3-6 July, 2019.

The symposium aims at bringing together world-leading experts in the fields of mechanobiology, cell biology and developmental biology studying the mechanical basis of cell and tissue morphogenesis. Particular emphasis was given to quantitative approaches analysing how force production, transduction and reception drives cell and tissue morphogenesis from the molecular scale to the organismal scale. The Symposium aims to provide a comprehensive overview of both experimental and theoretical advances providing insight into the molecular, cellular and biophysical mechanisms by which cells, tissues and entire organisms take shape.

Mechanobiology at the alveolar epithelium: can you feel the force?

Prof Mark Griffiths spoke about his recent work at the plenary session (chaired by Dr Charlotte Dean) at this year’s Summer Meeting at the British Association for Lung Research. His talk entitled ‘Mechanobiology at the alveolar epithelium: can you feel the force?’ detailed recent work exploring mechanisms underlying alveolar development. The conference was held at Selwyn College Cambridge between 10-12 September 2019.

The theme of this years gathering for the respiratory scientist community was ‘Lung Injury & Repair’. The BALR caters for the respiratory scientist community, from basic to clinical, to provide a platform to exchange ideas, create collaborations, and further pulmonary research. 

Sek Shir also presented recent findings at her poster entitled “Disruption of The Planar Cell Polarity (PCP) Component Vangl2 Alters Cell Mechanics in Loop-tail Mice”.

Selwyn College Cambridge

Planar Cell Polarity in Development across Evolution

One day meeting on 26th July ,2019

The Society for Developmental Biology Satellite Symposium hosted the first global specialist PCP meeting since 2013 on ‘Planar Cell Polarity in Development across evolution’

The talks spanned all aspects of PCP biology across Drosophila, Zebrafish, mouse and human.

Charlotte Dean’s talk, ‘The PCP pathway in Lung Development and repair’. highlighted the importance of the PCP pathway for proper lung morphogenesis during embryonic development. It also summarised more recent data looking at the role of Vangl2 in the adult lungs.  Making use of Vangl2Lp heterozygotes, which are viable as adults, Charlotte described how dysfunction of the PCP pathway lead to significant morphological and functional defects in the adult lungs, highlighting a role for the PCP pathway beyond embryonic development. This correlates with their findings that in the human population, individuals with a particular Vangl2 SNP who smoke, have significantly lower lung function compared to smokers without this SNP.  

 Legal seafood and Boston Public library

Stem cells, therapies and bioengineering in Vermont

July 15-18 2019, University of Vermont, Burlington, Vermont, USA.

The International Society for Cell & Gene Therapy hosted the bi-annual invitational Conference on Stem cells, Cell therapies and bioengineering in lung biology and disease

This meeting brings together scientists working on a variety of different regenerative medicine approaches to treat lung disease. The meeting covered advances in discovery biology, 3D models for lung research, technology and current approaches being trialed as potential treatments for lung disease.  Charlotte presented an invited talk entitled ‘Live imaging of alveologenesis in Precision-Cut lung slices reveals dynamic epithelial cell behaviour’.

Views of the beautiful Lake Champlain and Burlington